Composite
folKE + b0

Part:BBa_K137054:Design

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-07-28)


Constitutive folKE expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FolKE has been shown to be a good target for metabolically engineering the overexpression of folate in bacteria, particularly in L.lactis. One possible explanation for this is that since GTP cyclohydrolase I is involved in the first enzymatic step in the folate biosynthesis pathway, overexpression of this enzyme will increase the total flux through the pathway.


Source

The original folKE gene was extracted from the L.lactis subspe. IL1403 genome.

References